Journal: bioRxiv

Article Title: Contaminant Spot Check and Removal Assay (ContamSPOT) for Mass Spectrometry Analysis

doi: 10.1101/2024.01.05.574392

Figure Lengend Snippet: (A) Fluorescence spectra of 0.1% Triton, 0.1% Triton-Dye A43 complex, and peptides from BSA digest. (B) Contaminant screening of common detergents used in lysis buffer. 1% concentration was used for each detergent. (C) Nonlinear calibration curves for positively tested detergents in ContamSPOT fluorometric assay.

Article Snippet: A Fluorescence Imager (Bio-Rad ChemiDoc TM MP) was used to image the plate at 535 nm, followed by quantification in ImageJ.

Techniques: Fluorescence, Lysis, Concentration Assay

Journal: bioRxiv

Article Title: Contaminant Spot Check and Removal Assay (ContamSPOT) for Mass Spectrometry Analysis

doi: 10.1101/2024.01.05.574392

Figure Lengend Snippet: (A) ContamSPOT colorimetric (left) and fluorescence (right) assay screening of the extractants from ethyl acetate LLE. (B) SDS concentrations and peptide recoveries using different amount of ethyl acetate (fold of volume to samples) with three wash cycles. (C) Triton concentration after multiple wash cycles with 10-fold ethyl acetate volume. (D) Drying down under acidic condition improved the removal of volatile salt ammonium bicarbonate. (E) SDS concentrations and peptide recoveries after pure ethyl acetate LLE and water saturated (W.S.) ethyl acetate. (F) Scatter plot showing peptide intensity correlation before and after LLE cleanup. (G) Venn diagram of number of quantified proteins before and after LLE cleanup with two technical replicates.

Article Snippet: A Fluorescence Imager (Bio-Rad ChemiDoc TM MP) was used to image the plate at 535 nm, followed by quantification in ImageJ.

Techniques: Fluorescence, Concentration Assay

Journal: bioRxiv

Article Title: Contaminant Spot Check and Removal Assay (ContamSPOT) for Mass Spectrometry Analysis

doi: 10.1101/2024.01.05.574392

Figure Lengend Snippet: (A) ContamSPOT colorimetric measurement of SDS concentrations after various sample preparation methods. Sat. indicates saturated signal. (B) ContamSPOT fluorescence measurement of Triton concentrations. (C) Number of quantified proteins and peptides from different methods. (D) Venn diagram of reproducibly quantified proteins in different methods. (E) Principal component analysis of different methods with three replicates. (F) Violin plot showing coefficient of variance of different methods.

Article Snippet: A Fluorescence Imager (Bio-Rad ChemiDoc TM MP) was used to image the plate at 535 nm, followed by quantification in ImageJ.

Techniques: Sample Prep, Fluorescence